Plant Gene Resources of Canada:
Professional Staff

Dr. Lone Buchwaldt Lone Buchwaldt, Ph. D.

Plant Pathologist
Plant Gene Resources of Canada
Agriculture and Agri-Food Canada
Saskatoon Research and Development Centre
107 Science Place
Saskatoon, Saskatchewan S7N 0X2

Tel.: (306) 385-9417
Fax: (306) 385-9489
E-mail: Lone Buchwaldt

Current Research Activities

  • Disease resistance in pulse crops.
    Germplasm of agricultural crop species relevant to Canada, and their wild relatives, are being acquired from around the world particularly from international gene banks, research scientists, and plant breeders, and occasionally during collection trips in other countries. Accessions of selected crop species are being evaluated for resistance to plant pathogens important to the Canadian agricultural sector. Currently, accessions of lentil (Lens culinaris) are screened for resistance to anthracnose (Colletotrichum truncatum ) and ascochyta blight (Ascochyta lentis), and accessions of chickpea (Circer ann ) for resistance to ascochyta blight (Ascochyta rabiei). The number of resistance genes (their dominance and additivity) is being determined in the most resistant accessions for utilisation in plant breeding programs that will address serious production problems caused by the pathogens. In collaboration with other research scientists, molecular markers linked to disease resistance genes have been developed and linkage maps were updated.
     
  • Resistance to Sclerotinia sclerotiorum in various crop species.
    Elucidation of resistance to sclerotinia white mould in Brassica napus (canola) is underway in a collaborative effort between staff at PGRC and the Molecular Genetics Section at the Saskatoon Research Centre which is partially funded by private and public canola breeding companies. The work is based on a B. napus cultivar with high level of resistance to sclerotinia stem rot identified in China. Genetic studies of inheritance and development of microsatellite markers linked to QTLs conferring sclerotinia resistance are in progress. Accessions of common bean(Phaseolus vulgaris) and wild sunflower (Helianthus sp.) are being evaluated for resistance to S. sclerotiorum in field nurseries.
     
  • Research before 2001.
    Previous studies included integrated control of foliar diseases in pulse crops, lentil, chickpea and pea, using partial resistance to foliar pathogens combined with application of either contact or systemic fungicides at different rates and growth stages. A fungicide decisions support system was developed and tested in lentil for control of anthracnose and ascochyta blight in Saskatchewan and Manitoba.

Academic education and employment

  • M. Sc. and Ph. D. Agricultural Sciences, the Royal Veterinary and Agricultural University, Copenhagen, Denmark.
  • Post Doctoral Fellow / Research Associate, Department of Plant Science, University of Manitoba, Winnipeg.
  • Research Scientist, Forage Section, Agriculture and Agri-Food Canada, Saskatoon Research and Development Centre.
  • Biologist, Molecular Genetics Section, Agriculture and Agri-Food Canada, Saskatoon Research and Development Centre.

Memberships

  • The Canadian Phytopathological Society (CPS), 1995 - present.
  • Executive Board of the Canadian Phytopathological Society, 1997-2001.
  • Plant Disease Sub-Council of Saskatchewan, 1999 - present.
  • The Prairie Registration Recommending Committee on Grain (PRRCG),1996 - present.
  • Local Arrangements Committee for the 68th Annual Meeting of CPS, Winnipeg, 1995-97.
  • The Danish Phytopathological Society, 1998-91.
  • The European Coordination Group for Research on Pest Control in Oilseed Rape, 1982-90.

Financial support for research

  • Agricultural Development Fund (Saskatchewan)
  • Agri Food Innovation Fund (Saskatchewan)
  • Agro-chemical companies (Canadian)
  • Matching Investment Initiative (Federal Government)
  • Manitoba Pulse Growers
  • Plant-breeding companies (International)
  • Saskatchewan Pulse Growers
  • Western Grains Research Foundation (Western Canada)

Selected scientific publications

  • Li, R., Rimmer, R., Buchwaldt, L., Sharpe, A.G., Séguin-Swartz, G., and Hegedus, D.D. (2003) Interaction of Sclerotinia sclerotiorum with Brassica napus: Cloning and characterization of endo- and exo-polygalacturonases expressed during saprophytic and parasitic modes. Fungal Genet. Biol. (submitted)

  • Li, R., Rimmer, R., Buchwaldt, L., Sharpe, A.G., Séguin-Swartz, G., and Hegedus, D.D. (2003) Interaction of Sclerotinia sclerotiorum with a Resistant Brassica napus Cultivar: Expressed Sequence Tag Analysis Identifies Genes Associated with Fungal Pathogenesis. Fungal Genet. Biol. (submitted)

  • Tar'an, B., Buchaldt, L., Tullu, A, Banniza, S., Warkentin, T., and Vandenberg, B., 2003. Using molecular markers to pyramid genes for resistance to ascochyta blight and anthracnose in lentil (Lens culinaris Medik.). Euphytica (submitted)

  • Buchwaldt, L., Anderson, K., Morrall, R.A.A., Bernier, C.C., and Gossen, B, D. 2003. Identification of lentil germplasm resistant to Colletotrichum truncatum and characterization of two pathogen races. Phytopathology (accepted)

  • Chongo, C., Buchwaldt, L. Gosses, B.D., Lafond, G. P., May, W.E., Johnson, E. N., Hogg, T., 2003. Foliar fungicides to manage ascochyta blight (Ascochyta rabiei) of chickpea in Canada. Can. J. Plant Pathol. 25: 135-142.

  • Tullu, A, Buchwaldt, L., Warkentin, T., Taran, B., Vandenberg, A. 2002. Genetics of resistance to anthacnose and identification of AFLP and RAPD markers linked to the resistance gene in PI320937 germplasm of lentil (Lens culinaris Medikus). TAG 106 (3): 428-434.

  • Chongo, G., Bernier, C. C., and Buchwaldt, L. 1999. Control of anthracnose in lentil using partial resistance and fungicide applications. Can. J. Plant Path. 21: 16-22.

  • Buchwaldt, L., Morrall, R.A.A., Chongo, G., and Bernier, C.C. 1996. Windborne dispersal of Colletotrichum truncatum and survival in infested lentil debris. Phytopathology 86: 1193-1198.

Books and chapters

  • Rimmer, S.R. ,Schattuck, V.I., and Buchwaldt, L. Compendium on Brassica Crop Diseases. The American Phytopathological Society. (accepted)

  • Rimmer, S.R. and Buchwaldt, L., 1995. Diseases. In Brassica Oilseeds Production and Utilization. Eds. D. Kimber and D.I.. McGregor. CAB International p. 111-140.

Cultivars

  • CDC Glamis lentil. Vandenberg, A., Kiehn, F. A., Vera, C., Gaudiel, R., Buchwaldt, L., Dueck, S., Morrall, R. A. A. Wahab, J., and Slinkard, A. E 2002. Canadian Journal of Plant Science 82:103-104.
     
  • CDC Grandora lentil. Vandenberg, A., Kiehn, F. A., Vera, C., Gaudiel, R., Buchwaldt, L., Dueck, S., Morrall, R. A. A. Wahab, J., and Slinkard, A. E 2002. Canadian Journal of Plant Science 82:105-106.
     
  • CDC Sovereign lentil. Vandenberg, A., Kiehn, F. A., Vera, C., Gaudiel, R., Buchwaldt, L., Dueck, S., Morrall, R. A. A. Wahab, J., and Slinkard, A.E 2002. Canadian Journal of Plant Science 82:106-107.
     
  • CDC Vantage lentil. Vandenberg, A., Kiehn, F. A., Vera, C., Gaudiel, R., Buchwaldt, L., Dueck, S., Morrall, R. A. A. Wahab, J., and Slinkard, A. E 2002. Canadian Journal of Plant Science 82:109-110.
     
  • CDC Robin lentil. Vandenberg, A., Kiehn, F. A., Vera, C., Gaudiel, R., Buchwaldt, L., Dueck, S., Wahab, J., and Slinkard, A. E 2002. Canadian Journal of Plant Science 82:111-112.

Selected abstracts and proceedings

  • Buchwaldt, L. and Richards, K. W. 2003. Plant Gene Resources of Canada and the Canadian Plant Germplasm System. Can. J. Plant Pathol. (In press).
     
  • Buchwaldt, L, Yu, F. Q., Rimmer, S. R., Hegedus, D. J., 2003. Resistance to Sclerotinia sclerotiorum in a Chinese Brassica napus cultivar. 8th International Congress of plant pathology, Christchurch, New Zealand, p. 289 (abstr.).
     
  • Tullu, A., Breitkreutz, C., Tar'an, B., Barlow, B., Buchwaldt, L., Warkentin, T., Vandenberg, A. 2002. Construction of a linkage map and mapping of the gene for anthracnose resistance in lentil (Lens culinaris Medik). 4th Pulse Crop Research Workshop Edmonton, Alberta, p. 34 (abstr.).
     
  • Tar'an, B., Buchwaldt, L, Banniza, S., Tullu, A., Warkentin, T., and Vandenberg, A., 2002. Pyramiding of genes for resistance to ascochyta blight and anthracnose in lentil. 4th Pulse Crop Research Workshop Edmonton, Alberta, p. 53 (abstr.).
     
  • Diederichsen, A., Banniza, S., Buchwaldt, L., Vandenberg, A., Warkentin, T., and Richards, K. 2002. Characterization, evaluation and acquisition of pulse germplasm by Plant Gene Resources of Canada. 4th Pulse Crop Research Workshop Edmonton, Alberta, p. 40 (abstr.).
     
  • Tullu, A., Buchwaldt, L., Warkentin, T., and Vandenberg, A. 2001. Inheritance of resistance to anthracnose and marker identification for resistance in lentil (Lens culinaris Medik.). North American Pulse Improvement Association, North Dakota, p. 11 (abstr.).
     
  • Tar'an, B., Buchwaldt, L., Vandenberg, A., Warkentin, T., and Eujayl, I. 2001. Genetic study of resistance to ascochyta blight in lentil (Lens culinaris Medik). North American Pulse Improvement Association, North Dakota, p. 15 (abstr.).
     
  • Chongo, G., Gossen, B.D., Buchwladt, L., Adikari, T., and Rimmer, S.R. 2001. Assessing diversity in a Canadian population of Ascochyta rabiei of chickpea. North American Pulse Improvement Association, North Dakota, p. 18 (abstr.).
     
  • Buchwaldt, L, Vandenberg, A., Tullu, A., and Bernier, C.C. 2001. Genetics of resistance to anthracnose (Colletotrichum truncatum ) in lentil. 4th European Conference on Grain Legumes. Cracow, Poland, p. 242.
     
  • Chongo, G., Gossen, B.D., and Buchwaldt, L., 2000. Influence of plant age on resistance to Ascochyta rabiei in chickpea cultivars. Soils and Crops 2000, Saskatoon, p.164-166.
     
  • Anderson, K., Buchwaldt, L., Sillero, J., and Gossen, B. D. 2000. Host range of Colletotrichum truncatum from lentil. Can. J. Plant Pathol. 22: 180 (abstr.).
     
  • Buchwaldt, L. and Bernier, C.C. 1998. Genetic study of resistance in lentil (Lens culinaris) to Colletotrichum truncatum the cause of anthracnose. Can. J. Plant Path. 20: 120 (abstr.)

Research-letters

  • Gossen, B.D., Buchwaldt, L., Chongo, G., and Armstrong, C. 2001. Ascochyta blight of chickpea. AAFC, SRC, Research letter 01-02, May 11, 2001, 2 pages.

  • Buchwaldt, L., Gossen, B.D., and Pearse, P. 2001. Ascochyta blight of lentil. AAFC, SRC, Research letter 01-03, May 22, 2001, 2 pages.

  • Pearse, P., McVicar, R., Goodwillie, D., Gossen, B., Buchwaldt, L., Chongo, G. and Armstrong, C. 2001. Ascochyta blight of chickpea. Farm Facts, Saskatchewan Agriculture and Food, 4 pages.

  • Chongo, G., Gossen, B.D., Armstrong, C., and Buchwaldt, L. 2000. "Resistant" chickpea are susceptible to ascochyta blight at flowering. AAFC, SRC, Research Letter 00-04, 2 pages.

  • Buchwaldt, L., Chongo, G., and Gossen, B.D. 1998. Management of lentil anthracnose. Agriculture and Agri-Food Canada, Fact sheet, 2 pages.

Web site

Pest Management Research Reports

  • Buchwaldt, L., Gossen, B.D., Hwang, S.F., Kutcher, R., and Chongo, G. 1999. Effect of foliar fungicide application on control of Mycosphaerella blight in pea, 1999. Pest Management Research Report: 288-290.

  • Chongo, G., Gossen, B.D., and Buchwaldt, L. 1999. Fungicidal control of ascochyta blight in chickpea in 1999. Pest Management Research Report: 275-276.

  • Chongo, G., Gossen, B.D., and Buchwaldt, L. 1999. Effect of Bravo and Quadris on ascochyta blight and yield of chickpea in 1998. Pest Management Research Report: 272-274.

  • Buchwaldt, L., Gossen, B.D., and Chongo, G. 1999. Effect of foliar fungicide application for control of diseases in lentil 1999. Pest Management Research Report: 277-279.

  • Buchwaldt, L., Gossen, B.D., Hwang, S.F., Kutcher, R., Turkington, K., and Xue, A. 1998. Effect of foliar fungicide application for control of Mycosphaerella blight in pea. Pest Management Research Report: 268-271.

  • Buchwaldt, L., Gossen, B.D., and Chongo, G. 1998. Effect of foliar fungicide application for control of anthracnose in lentil. Pest Management Research Report: 283-284.

Plant Disease Surveys

  • Chongo, G., Buchwaldt, L., Anderson, K., and Gossen, B.D. 2000. Saskatchewan chickpea disease survey - 1999. Can. Plant Dis. Surv.: 86-97.

  • Anderson, K. L., Buchwaldt, L., Chongo, G., Gossen, B. D. , Morrall, R.A.A., and Pearse, P. 2000. Diseases of lentil in Saskatchewan 1999. Can. Plant Dis. Surv. 96-98.

  • Buchwaldt, L., Anderson, K., and Gossen, B.D. 1999. Disease survey of lentil in Saskatchewan 1998. Can. Plant Dis. Surv: 126-127.

  • Buchwaldt, L., Bernier, C.C., and Platford R.G. 1993. Anthracnose and other diseases of lentil in Manitoba in 1992. Can. Plant. Dis. Surv. 73: 88-90.

  • Buchwaldt, L., Platford, R.G., and Bernier, C.C. 1992. Survey of anthracnose on lentil in Manitoba 1991. Can. Plant Dis. Surv. 72: 78-79.

Articles in producer magazines

  • Buchwaldt, L. and Gossen, B.D. 2001. To spray or not to spray: assessing disease risk in lentil. Saskatchewan Pulse Growers Newsletter Vol. 17 (2): 1-4.

  • Buchwaldt, L. and Gossen, B.D. 2001. Disease risk assessment in lentil. Saskatchewan Pulse Growers Newsletter, Vol. 17 (x): 16-17.

  • Buchwaldt, L. and Gossen, B.D. 2000. When to spray fungicides in lentil. The Saskatchewan Pulse Grower Vol. 16: 1-7.

  • Buchwaldt, L. and Denys, S. 1999. Tips for anthracnose and ascochyta control in lentils. The Western Producer, Farming. Vol. 2(6): 27-28.

  • Buchwaldt, L. and Gossen, B.D. 1999. When to spray fungicides in lentil and pea crops. The Saskatchewan Pulse Grower Vol. 15 (2): 1, 6-7.

  • Buchwaldt, L. 1999. Handling of anthracnose-infected lentil stubble: Avoid tillage. Saskatchewan Pulse Grower Vol. 15 (3): 15.

  • Buchwaldt, L. and Bernier, C.C. 1997. Lentil anthracnose is spread by wind. Pulse Beat (20): 10 -11.

  • Bernier, C.C., Buchwaldt, L., Chongo, G., and McCallum, B. 1995. We are getting closer to achieving control of anthracnose in lentil. The Pulse Beat (15): 21-23.

  • Buchwaldt, L., Chongo, G., and Bernier, C.C. 1994. First year field test of a lentil line with moderate resistance to anthracnose in combination with application of Bravo 500. The Pulse Beat (11): 20.

  • Buchwaldt, L., Bernier, C.C., and Morrall, R.A.A. 1993. Anthracnose on lentil - A summary of 3 year's Research. The Pulse Beat (7): 24-29.

  • Buchwaldt, L. and Bernier, C.C. 1993. The University of Manitoba 1993, field trials in lentil for control of anthracnose. The Pulse Beat (5): 18-19.

  • Buchwaldt, L., Bernier, C.C., and Morrall, R.A.A. 1993. Evaluation of Bravo and Tilt for control of anthracnose in lentil 1992. The Pulse Beat (3): 22-24.

  • Buchwaldt, L. and Bernier, C.C. 1993. Anthracnose on lentil 1992 - A summary of ongoing research. The Pulse Beat (3): 25-26.